![]() Download a list of TrueBlack® references.įluorescent dyes can be an unexpected cause of non-specific antibody binding It also has been used to quench fluorescence on polycarbonate filters used as cell supports for imaging ( Futia et al. It is not as effective at quenching these sources of autofluorescence as it is for lipofuscin, but it can improve background in human and non-human tissue types. TrueBlack® also can reduce autofluorescence from other sources, such as collagen, elastin, and red blood cells (Figures 4-5). TrueBlack® effectively eliminates lipofuscin autofluorescence in tissues like human brain and retina. TrueBlack® reduces autofluorescence from other sources too See TrueBlack® FAQs for more information. Quenching is stable and compatible with commonly used wet-set and hardset fluorescence mounting media, so slides can be stored after staining. It is rapid, simple, and has minimal effect on signal from fluorescent antibodies or nuclear dyes, thus preserving specific staining. TrueBlack® treatment can be performed before or after immunostaining (Figures 2-3).
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